af488 goat anti human iga (Jackson Immuno)
Structured Review
Af488 Goat Anti Human Iga, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iga+af488/Alexa+Fluor+488+AffiniPure+Goat+Anti-Human+IgA+%2B+IgG+%2B+IgM/us11834493-603-28-32
Average 93 stars, based on 5 article reviews
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Binding Assay:Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies Article Snippet: .. Cultures containing antibodies with binding-profile-of-interest were selected, washed with MACS buffer (PBS with 5% FBS and 2 mM EDTA), and stained with CD19-PE-Cy7 (BD, catalog no. 341113, 1:100), IgM-AF647 (Jackson Immuno, catalog no. 109-606-129, 1:500) and Magnetic Cell Separation:Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies Article Snippet: .. Cultures containing antibodies with binding-profile-of-interest were selected, washed with MACS buffer (PBS with 5% FBS and 2 mM EDTA), and stained with CD19-PE-Cy7 (BD, catalog no. 341113, 1:100), IgM-AF647 (Jackson Immuno, catalog no. 109-606-129, 1:500) and Staining:Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies Article Snippet: .. Cultures containing antibodies with binding-profile-of-interest were selected, washed with MACS buffer (PBS with 5% FBS and 2 mM EDTA), and stained with CD19-PE-Cy7 (BD, catalog no. 341113, 1:100), IgM-AF647 (Jackson Immuno, catalog no. 109-606-129, 1:500) and other:Article Title: ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies Article Snippet: Cells were stimulated with 2.5 μg/ml of R848 (Invivogen, catalog no. tlrl-r848-5) in complete medium (RPMI 1640 medium (catalog no. 31870-025) supplemented with 2 mM glutamine (catalog no. 35050-038), 1% (vol/vol) nonessential amino acids (catalog no. 11140050, 1% (vol/vol) sodium pyruvate (catalog no. 11360-039), PenStrep (50 U/ml penicillin, 50 μg/ml streptomycin, catalog no. 15070-063), Kanamycin (50 U/ml, catalog no. 15160-047), 0.1% beta-mercaptoethanol (catalog no. 31350- 010) (all from Gibco), 10% Hyclone (Cytiva, catalog no. SH30070.03), 0.5% Transferrin (30 μg/ml) (LubioScience, catalog no. 0905-100), and 1,000 U/ml IL-2 (produced in house from transfected J558L cells). |
